Spectrophotometers

Over what wavelength range would you like to measure samples?

SPECTROPHOTOMETERS

UV/Visible
Often abbreviated to UV/Vis or UV-Vis. UV/Visible spectroscopy offers maximum flexibility and is suitable for applications in the wavelength range of 190 to 1100nm. In UV/Visible spectroscopy the UV region is considered to be any wavelength less than 340nm. Nucleic acid, purified proteins, and other organic molecules are often measured in the UV region.

Visible
Often abbreviated to Vis. Visible spectrophotometers and colorimeters typically measure in the visible region of the electromagnetic spectrum (340-750nm). If your samples are colored this is an indication that they will absorb visible light. If you do not need to measure in the UV region (<340nm) a visible spectrophotometer or colorimeter may be a more economical alternative.

MRC's spectrophotometers UV/vis, vis with many options like Kinetics, Scanning, DNA protein Multi wavelengths, etc.

Single Beam Spectrophotometer: SPECTROPHOTOMETERS

 Types of Spectrophotometers

-Fluorescence spectrophotometer

-Atomic absorption spectrophotometer.

-Micro Spectrophotometer

-Visible Spectrophotometer

-UV-VIS spectrophotometer

UV-Visible Spectrophotometers

These workhorse instruments cover a broad spectrum of applications, particularly in chemistry and biology. They are adept at analyzing substances that absorb UV or visible light, making them indispensable in laboratories worldwide.

Infrared Spectrophotometers

Delving into the invisible realm, infrared spectrophotometers are vital for studying molecular vibrations. Widely used in pharmaceutical and organic chemistry, they offer insights beyond the capabilities of UV-Visible counterparts.

Fluorescence Spectrophotometers

These specialized instruments excel in detecting fluorescence emitted by certain compounds. Widely used in biochemistry and molecular biology, they are invaluable for studying complex biological systems.

Applications of Spectrophotometer

-Enzyme assay

-To Discovery the molecular weight of a particular sample

-Qualitative analysis

-To set the unknown concentration of a given species through absorption spectrometry.

-Characteristics of a protein

Working Principle

At its core, spectrophotometry revolves around the interaction of light with matter. The instrument measures the amount of light absorbed or emitted by a substance, unveiling valuable information about its properties. Understanding this fundamental principle is crucial for grasping the versatility of spectrophotometers.