
Homogenization is a very common sample preparation step
prior to the analysis of nucleic acids, proteins,
cells, metabolism, pathogens, and many other targets.
High-shear mechanical methods for cell disruption work
by placing the bulk aqueous media under shear forces that literally pull the
cells apart. These systems are especially useful for large scale laboratory
experiments (over 20 mL) and offer the option for large-scale production.
Factors to consider when selecting a homogenizer
·
Wet or Dry Sample
·
Sample Size
·
Generator Width
·
Location of Generator within the Sample
·
Speed and Particle Size Reduction
Most homogenizing processes can be done in 15 - 30 seconds.
If not you may be:
·
Using the wrong size generator
·
Not locating the generator properly in
the container
·
Too viscous of a material