Autoclave uses pressurized steam to destroy all of its antimicrobial life, which may be on a contaminated item. For this reason, autoclaves are commonly used in laboratories, operating rooms in hospitals and food production facilities, in order to sterilize products, tools, and instruments.
Autoclave Instructions-Guide
Before operating the Autoclave, all laboratory personnel must perform training on the device and read the manufacturer's instructions.
Some of the training you should know:
-Proper safety protocol.
-How to make sterilization items.
-How to safely load and unload materials from an autoclave.
-How to select the appropriate cycle according to the sterilized item.
-Automatic operating procedure.
It is recommended to wear the following while using:
-Closed shoes
-safety glasses
- Heat resistant hand gloves
-lab coat
This equipment is designed to protect the operator from burns, boiling liquids and other injuries.
Loading
It is important that the operator never place sealed containers in the device. Because the pressure is inside the autoclave, it can cause glassware to explode. Also, never put items containing solvents in the autoclave, as these can emit toxic fumes when heated. Always properly inspect the tools and products that are inserted into the device.
Sterilization time
The sterilization time will vary depending on a number of factors, including the type of material, the size of the load, the type of load and the cycle you choose. If you are not sure how long to operate the device, consult the manufacturer to determine the best type of cycle and operating time.
Cycle selection
-If you are using an autoclave with an upward-opening door, close the door completely and rotate the locking mechanism until hermetically locked. Be careful not to apply too much force to the locking mechanism, as this may damage or break the internal locking mechanism of the door lock.
-Select the correct program, depending on the type of material or tool inserted into the cell.
-When disinfecting liquids, it is mandatory to use a liquid-only program, otherwise a blockage may occur in the device.
Unloading and finishing work
-After completing the cycle and pressure inside the device, make sure no one is standing near the unit and open the door slightly and carefully. Wait for the steam to exit and only then lift the door and leave it open. Then take the tools out.
-If there is a chemical fume hood above the autoclave, make sure it is turned on while the door is open and the steam inside the room is coming out.
-Allow the steam to dissipate and the dishes to cool for about 10 minutes.
Operating the Autoclave
Check the water level in the autoclave.
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Load materials properly.
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Set the cycle (usually 121°C for 15-30 minutes).
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Start the run and monitor.
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Wait for pressure release before opening the door.
Setting the Right Time, Temperature, and Pressure
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121°C at 15 psi for most items
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Adjust time for volume: large liquid loads need more time
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134°C cycles may be used for surgical tools
Loading the Chamber Properly
Don't overload it! Leave space between items. Point bottle caps slightly open (never screw them shut). Let steam move freely.
This article is written to understand in a basic way, how to operate an autoclave and how to maintain safety.
Before using the device, it is mandatory to read the device manufacturer's and safety instructions as recorded in the operating instructions.
