Important issues to notice when performing centrifugal separations: Centrifugation data should be stated either in units of Relative Centrifugal Force (RCF) or gravities (g), or the data should be stated so RCF can be calculated using revolutions per minute (RPM) and the dimensions of the centrifuge rotor. Centrifugation at the same RPM and duration will exert different centrifugal forces on samples if centrifuge rotors having different radius sizes, bucket types or bucket sizes are used. Different centrifugal forces produce different sample separation rates at the same RPM and duration. In order to reproduce the experimental conditions when a different type or make of rotor is to be used, it is essential to know the precise dimensions and characteristics of each rotor. If the RPM and rotor radius is known, a monogram chart can be used to determine the RCF. RCF (or g) can be calculated by using the following formula (Rickwood, 1984, p. 288):
RCF=11.18×r×(N/1000)²
r = the radius in centimeters from the center of the rotor to the sample during centrifugation
N = the rotor speed in RPM
Read more about Centrifugal Force
PPP Plasma Gel Making Process
What is Principle operation of Centrifuge?
The principle of centrifuge can be explained in the same way as sedimentation can be. The reason behind this is the difference in densities. Concerning density, the lighter ones float on top of the container while the denser particles sink.
Types of Centrifuge:
• Low speed Centrifuges
• Ultra centrifuge
• High speed Centrifuges
• Microcentrifuge
• Benchtop centrifuge
• Refrigerated
• Floor-standing Centrifuges
• Blood Centrifuges
• Hematocrit Centrifuges
• Clinical PRP/PRF Centrifuges
Application of Centrifuges
-To separate fluids, gases, or liquids
-Used for cell, organelle, virus, protein, and nucleic acid purification.
-Separation of blood components.(serum or plasma)
-Separation of mixtures with close densities
-liquids separation
-Protein extraction
-Isotope
-DNA extraction
What industries are centrifuges for?
-Dentists
-Orthopedics
-Beauty salons
-Hair transplants
-Veterinarians
-Dialysis / nephrology institutes
- Blood and urine tests
-Hematology Labs, Immunology
CENTRIFUGES For Sale
MRC supply a wide range of Centrifuges. Different sizes and volumes. Contact us
